- Build the high-resolution microscope
- Build the low-cost microscope
- Build the upright microscope
- Customisations and alternatives
- Troubleshooting
- Knowledge base

# Assemble the basic optics module

The imaging optics for this version of the microscope consist of a Raspberry pi camera and the lens from the camera. To create a microscope, the wide angle lens is reversed and separated from the camera. This makes quite a good microscope objective with a field of view about 400μm across and a resolution of around 2μm.

## For this section you will need:

### Tools

- 1 #1 pozidrive screwdriver
- 1 Pi Camera lens tool - This should come with the Raspberry Pi Camera Module v2. If it is missing, you can 3D print a workaround lens remover.

### Printed Parts

- 1 Lens spacer
- 1 pi camera platform

### Electronic Components

- 1 300mm Pi Camera ribbon cable - This is longer than the standard ribbon cable the Pi Camera is sold with!
- 1 Raspberry Pi Camera Module v2

### Mechanical Components

- 1 M3 nut
- 1 M3x10 cap head screws
- 4 No 2 6.5mm self tapping screws

These instructions assume you will build the upright microscope with a basic optics module. You can also use the high resolution optics module (with an RMS objective) on the upright microscope, or other options from the customisations page.

If using the high resolution optics module, a 400mm Raspberry Pi ribbon cable is preferable due to the additional height.

## Step 1: Visually inspect the lens spacer

Take the lens spacer and confirm that:

- It has been printed in black
- It is dust free (You can blow air through to clean it)
- The central shaft is not obstructed by strings of plastic.

## Step 2: Remove Pi Camera Lens

Caution!

The camera board is static sensitive.

- Before touching the Pi Camera touch a metal earthed object. If you own one, consider wearing and anti-static strap.
- Take the Pi Camera out of the package. Make sure to hold it only by the sides of the board.
- Take the protective film off the lens.
- Take the Pi Camera lens tool and place it over the lens
- Slowly unscrew the lens (About 4 full turns of the tool)
- Carefully lift off the lens.
- Save the lens and the camera, we use both in this version of the microscope.

## Push-fit the lens

- Work out which side of the pi camera lens used to be facing the camera sensor (This is the side with more lens visible).
- Place the pi camera lens on a clean surface with the side that was next to the camera sensor on the bottom.
- Push the lens spacer down onto the lens until it clicks into place.

## Step 3: Attach the Pi Camera

- Take the Pi Camera and place it ontop of the pi camera platform.
- Place the lens spacer over the picamera
- Use four No 2 6.5mm self tapping screws to secure the three parts together using a #1 pozidrive screwdriver
- Take care to not over torque the screws.

## Step 4: Attach the mounting screw

- Take an M3 nut and push it into the nut trap from the top
- Take an M3x10 cap head screws and screw it into the nut.
- Only screw it in a couple of turns. About 5 mm of thread should still be visible

## Step 5: Connect ribbon cable

- Take the 300mm Pi Camera ribbon cable
- Pull the catch forward on the exposed Pi Camera connector
- Insert the ribbon cable with the contacts towards the board
- Close the catch on the connector

Set the complete optics module aside in a safe place.

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